Fig. 3

The chalky phenotype and physiochemical analysis in the N2/N3 mutants vs WT. All the characters in this figure are detected in full mature seeds (125 DAGs). a The chalky phenotypes and shape phenotypes of N2/N3 mut compared with WT. All the T2 mutants are planted in Fuyang (Hangzhou, 2021), n = 15 mm. There are obvious white-core and white-belly in N2 mutant which chalky area is the largest, while there is medium transparent in WT and white-core only in N3 mutant. b The physiochemical analysis of N2/N3 mutants is distinct compared with WT. The quality characters such as SSC, amylose content, protein content were tested and the results were as follows: The SSC was highest in N2 mutant but lowest in WT; The amylose content was lowerin N2/N3 mutants compared with WT, which was reversed to the SSC tendency in mutants; The protein content was lowest in N3 but no significant difference between WT and N2 (two-way ANOVA for mixed model, *, p < 0.05 and **, p < 0.01). c The hand-cut sections of full-mature endosperms are scanning by SEM (scale bar, 25 mm and 500 um). The starch granules arranged distinctly between N2 and N3 mut compared with WT, and the structure of starch granules are smaller and more polyhedral in mutants. d The amylopectin analysis shows the apparent difference in N2/N3 mut and WT. The chain length distribution of amylopectin analysis was calculated by the number of short chains with degree of polymerization (DP). There are more short fragments in N2 mut while more medium fragments in N3 mut