Fig. 4

The DEGs clusters and GO enrichment of RNA-Seq in mutant vs WT seeds (3 DAP) Down-regulated DEGs in Venn. a The Venn diagram of up–regulated DEGs are based on the RNA-Seq of seeds (3 DAP). b The Venn diagram of down–regulated DEGs based on the RNA-Seq of seeds (3 DAP). All the genes with label are listed in Table S5. In total, five mutants N2-1, N2-2,N2-z, N3-1,N3-3 were used for the cluster analysis, and the N2-2 and N3-1 duplicated samples are N2-z and N3-3. c The volcano cluster of the DEGs in RNA-Seq of seeds (3 DAPs). As cluster shown, most of the DEGs in red are up-regulated genes, the down-regulated genes are in blue and the genes expression with no difference are in yellow. All the genes expressed significantly are listed in Table S8. d The volcano cluster of the DEGs in Sucrose and Starch pathway of RNA-Seq in N2/N3 mut vs WT seeds (3DAPs). All significantly expressed genes are listed in Table S9