Fig. 6

The Mapman analysis of Sucrose and Starch metabolism pathway in seeds (3DAP) of N2 vs WT and qRT-PCR verification of the DEGs in RNA-Seq KEGG. a The Mapman analyses are totally based on the Sucrose and Starch metabolism. The DEGs are screened from the RNA-Seq of N2 vs WT seeds (3 DAPs). There are 29 ECs in this KEGG pathway, and the heatmap diagram in each EC contain four samples: WT, n2-1, n2-2, n3-1 (Fig. S8). This Mapman constructure shows a dynamic process related to source-sink balance during grain filling procession. b The qRT-PCR verifications of 39 genes significantly expressed in ECs of this MapMan, which is based on the RNA-Seq KEGG in N2 vs WT seeds (3DAPs). These 39 genes expressions in seeds (3DAP) are detected repeatedly by qRT-PCR in both N2 and N3 mut vs WT. The almost all DEGs in mutants are down-regulated while the RSUS1 (LOC_Os03g28330), OsBE2b (LOC_Os02g32660), endoglucanase 7 (LOC_Os02g50490), OsDPE2 (LOC_Os07g46790), phosphoglucomutase 2 (LOC_Os03g50480) are up-regulated in N2 mut significantly (Table S11)