Fig. 8

Expression and biological analysis of POLV and JMJ14-regulated genes that are also stimulated in wild-type (WT) plants. a Venn diagram including a list of A. thaliana genes that are possibly directly controlled by POLV, JMJ14, and repeated stress in WT plants. The POLV and JMJ14 sets included genes that were activated in polv or jmj14-infected mutants compared to WT infected plants at 4 and/or 7 days post-inoculation (dpi) and overlapped with specific epigenetic marks or proteins. The WT set consisted of genes that were regulated in TuMV-infected plants, when comparing plants that were previously stimulated with a ROS-tagged TuMV to plants that were only mock-inoculated. b Biological process types at intersections of genes regulated in WT and mutant plants. The Panther database was used for gene list analysis. C RT-qPCR study of selected genes in WT plants subjected to repeated viral stresses and control plants. Plants were either mock-inoculated or infected with a ROS-tagged TuMV, and the inoculated leaves were removed 26-28 hours later. Three days after leaf removal, plants were inoculated with TuMV. Mock samples were mock-inoculated before and after leaf removal. Unstimulated samples were mock-inoculated before leaf removal and subsequently inoculated with TuMV. Stimulated samples were TuMV-inoculated before leaf removal and then inoculated again with TuMV. Each dot represents a biological replicate, which consists of individual plants collected four or eight days following the TuMV challenge phase. Two of the six selected genes (LURP1-LIKE and RLP43) were predicted to be regulated by POLV during virus infection, whereas four (PP2-A5, RPW8, CDC48B, and PYL5) were predicted to be controlled by JMJ14. A. thaliana endogenous genes SAND and PP2A served as controls for the relative quantification. Bonferroni correction tests were used for t-test pairwise comparisons; ***P < 0.001; **P < 0.01; *P < 0.05; ns., not significant