Fig. 7From: Mathematical kinetic modelling followed by in vitro and in vivo assays reveal the bifunctional rice GTPCHII/DHBPS enzymes and demonstrate the key roles of OsRibA proteins in the vitamin B2 pathwayRiboflavin content in rice callus overexpressing AtRibA1 and OsRibA1 and OsRibA2. As negative controls, OsRibA1 and OsRibA2 were also used with a point mutation leading to an early stop codon (indicated as OsRibA1* and OsRibA2*). Twelve independent lines, positive for each transgene, were randomly pooled and stored at -80°C until riboflavin extraction. Riboflavin was detected and quantified by HPLC–MS. Data shown correspond to means ± standard deviation of the means (SD), N = 12. Data was compared to control values (WT) by One-way ANOVA (**, P < 0.01)Back to article page