Fig. 2
In-vitro screening of pearl millet panicle associated Bacillus isolates PBs 12 and PBl 36 against diverse fungal plant pathogens. Note: Dual culture assays to estimate the bio-control activity of bacterial strains associated with panicles of Cenchrus americanus, performed on sterile Potato Dextrose Agar plates with six taxonomically diverse fungal plant pathogens. 48 h old pure bacterial culture [column 3 (b); 3 (c)] used for bacterial suspension (1 × 107 cfu/ml) preparation. Loopful of culture steaked one side of the Petri plate (90 mm) and mycelial disc (4 mm dia.) placed opposite side confronting the bacteria. 3 replications maintained for each isolate and culture plates without bacteria and inoculated with only fungal pathogens served as mock [column: 3 (a))]. All the plates incubated at 28° C until the mycelium cover mock plate completely, then the inhibition zone measured and average of 3 replications considered as final radial mycelial growth reading. Experiment repeated twice to check the reproducibility