Comprehensive genomic analysis of Bacillus subtilis and Bacillus paralicheniformis associated with the pearl millet panicle reveals their antimicrobial potential against important plant pathogens

Table 2 Seed priming with pearl millet panicle associated Bacillus strains PBs 12 and PBl 36 to test their plant growth promotion activity

Isolate name	Shoot length	Root length	Germination percentage (%)	Seedling vigour index*
*Bacillus subtilis* PBs 12	6.87b	5.62b	95	1186.55
*Bacillus licheniformis* PBl 36	7.38a	6.42a	96	1324.80
Mock	5.01c	5.56b	95	1004.15
CD (p = 0.05%)	0.49	0.22	-	-

Data sharing the same letter designation in each column, indicate no significant difference between them at a significance level of p = 0.05
CD (Critical Difference): Smallest difference between treatments means likely to indicate true change
24 h old bacterial cultures of PBs12 and PBl 36 used to prepare 1 OD (1 × 10⁸ cfu/ml) cell suspension. Pearl millet cv. 7042S seeds (n = 100) were soaked overnight in cell suspensions and incubated for 7 days at 27^o C temperature and 98% relative humidity. Data recoded and interpreted 7 days after incubation
Seedling vigour index was calculated using the formula:
\(\mathrm{Seedling vigour index}* = [(\mathrm{Seedling length}) * (\mathrm{Germination percentage})]\)

ISSN: 1471-2229