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Fig. 2 | BMC Plant Biology

Fig. 2

From: Long-term organogenic callus cultivation of Ranunculus illyricus L.: a blueprint for sustainable ex situ conservation of the species in urban greenery

Fig. 2

Development of various explants of R. illyricus on B5 mineral medium with MS micronutrients and various PGR combinations after 6 weeks of cultivation in A) photoperiod or B-F) darkness. (A) Apical buds on medium supplemented with 3.2 mg L− 1 NAA and 5.0 mg L− 1 kinetin; callus on the surface of (B) leaf and (C) fragments of stolons; (D) receptacle on medium with 8.0 mg L− 1 picloram and 8.0 mg L− 1 kinetin; (E) developing tubers on fragments of stolons on medium with 4.0 mg L− 1 2,4-D and 8.0 mg L− 1 kinetin; (F) callus of stolon-origin on medium with 8.0 mg L− 1 picloram and 5.0 mg L− 1 kinetin, bar = 1 cm

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