Fig. 3

Regeneration of Ranunculus illyricus shoots in vitro. (A) section through stolon-origin callus with a differentiating adventitious bud after six weeks of cultivation on medium with 2.0 mg L^− 1 IBA and 4.0 mg L^− 1 BAP in darkness, Lp – leaf primordia, Cc - callus cell, Mz – meristematic zone, bar = 100 μm; (B) proliferating shoot bud after six weeks of cultivation on medium with 1.0 mg L^− 1 IBA and 2.0 mg L^− 1 BAP in photoperiod, bar = 1 cm; (C) cluster of adventitous buds/shoots with roots on the medium with 1.0 mg L^− 1 IBA and 2.0 mg L^− 1 BAP in photoperiod, bar = 1 cm 4.3. Multiplication and rooting of the shoots