Table 2 Types of initial explants and plant growth regulators used to initiate R. illyricus cultures in vitro
Type of explant | Auxins (mg L− 1) | Cytokinins (mg L− 1) | Number of explants |
|---|---|---|---|
Apical buds | NAA* 3.2 | Kinetin 5.0 | 7 |
NAA 2.5 | BAP** 2.5 | 4 | |
Leaf | Picloram 8.0 | Kinetin 3.0 | 6 |
Picloram 8.0 | Kinetin 5.0 | 22 | |
Picloram 8.0 | Kinetin 8.0 | 20 | |
NAA 5.0 | BAP 5.0 | 7 | |
NAA 2.5 | BAP 2.5 | 3 | |
Petioles | Picloram 8.0 | Kinetin 5.0 | 2 |
Picloram 8.0 | Kinetin 8.0 | 3 | |
NAA 5.0 | BAP 5.0 | 6 | |
NAA 2.5 | BAP 2.5 | 3 | |
Stolons | Picloram 8.0 | - | 10 |
Picloram 8.0 | Kinetin 3.0 | 13 | |
Picloram 8.0 | Kinetin 5.0 | 14 | |
Picloram 8.0 | Kinetin 8.0 | 14 | |
2,4-D*** 4.0 | Kinetin 8.0 | 11 | |
NAA 5.0 | BAP 5.0 | 5 | |
NAA 2.5 | BAP 2.5 | 5 | |
Flower receptacle | Picloram 8.0 | Kinetin 5.0 | 3 |
Picloram 8.0 | Kinetin 8.0 | 6 | |
Tubers | 2,4-D 4.0 | Kinetin 8.0 | 34 |