Table 4 Results of the decontamination and growth reaction of different kinds of R. illyricus explants on media with different compositions of plant growth regulators
Type of explant | Auxins (mg L− 1) | Cytokinins (mg L− 1) | % of explants disinfected per isolated explants | % of callusing or growing* explants |
|---|---|---|---|---|
Apical buds | NAA 3.2 | Kinetin 5.0 | 100** | 100 |
NAA 2.5 | BAP 2.5 | 75** | 100 | |
Percentage of disinfection | 87.5% | Â | ||
Leaf | Picloram 8.0 | Kinetin 3.0 | 50 | 0 |
Picloram 8.0 | Kinetin 5.0 | 82 | 50 | |
Picloram 8.0 | Kinetin 8.0 | 90 | 80 | |
NAA 5.0 | BAP 5.0 | 100 | 28 | |
NAA 2.5 | BAP 2.5 | 100 | 0 | |
Percentage of disinfection | 84% | Â | ||
Petioles | Picloram 8.0 | Kinetin 5.0 | 50 | 50 |
Picloram 8.0 | Kinetin 8.0 | 100 | 100 | |
NAA 5.0 | BAP 5.0 | 83 | 33 | |
NAA 2.5 | BAP 2.5 | 67 | 50 | |
Percentage of disinfection | 75% | Â | ||
Stolons | Picloram 8.0 | - | 80 | 80 |
Picloram 8.0 | Kinetin 3.0 | 85 | 54 | |
Picloram 8.0 | Kinetin 5.0 | 86 | 86 | |
Picloram 8.0 | Kinetin 8.0 | 71 | 71 | |
2,4-D 4.0 | Kinetin 8.0 | 73 | 36 | |
NAA 5.0 | BAP 5.0 | 100 | 0 | |
NAA 2.5 | BAP 2.5 | 100 | 0 | |
Percentage of disinfection | 85% | Â | ||
Flower receptacle | Picloram 8.0 | Kinetin 5.0 | 100 | 0 |
Picloram 5.0 | Kinetin 8.0 | 100 | 0 | |
Percentage of disinfection | 100% | Â | ||
Tubers | 2,4-D 4.0 | Kinetin 8.0 | 100 | 0 |
Percentage of disinfection | 100% | Â | ||