Fig. 2

Fine mapping, gene discovery (LLSR1 gene for LLS resistance), marker development and validation of major main-effect QTLs controlling LLS resistance. (a) The 2.08 Mb genomic region on chromosome A02 wherein QTLs were detected across different seasons, with eight candidate resistance genes targeted for marker development. The gene IDs for these candidate resistance genes and KASP assay information are also provided. (b) Scatter plot showing the validation results for selected KASP assays (snpAH0003, snpAH0004, snpAH0005, snpAH0003, snpAH0010, snpAH0011, snpAH0014, snpAH0120, and snpAH0121). There are three clusters on each scatter plot. The red cluster indicates the resistant lines, the blue cluster indicates the susceptible lines, and the green clusters are the heterozygotes derived from the crosses between the resistant and susceptible lines