Table 2 Major QTLs targeted for candidate gene discovery and KASP assay development for late leaf spot (LLS), leaf rust and high oleic acid content
Trait | Gene/Marker interval | Position (cM) | LOD | PVE (%) | Additive effect | KASP assays |
|---|---|---|---|---|---|---|
LLS70, LLS80, LLS90 | S2_976663 - S2_454537 | 2.01 | 11.69–29.97 | 16.3–44.2 | (-0.584) to (-0.498) | snpAH0010, snpAH0011, snpAH0120, snpAH0121, snpAH0122, snpAH0123, snpAH0126 |
LLS70, LLS90 | S2_1228446 - S2_1281741 | 3.71 | 9.3-13.97 | 17.3–23.9 | (-0.537) to (-0.664) | snpAH0004, snpAH0005 |
LLS70 | S2_2808680 - S2_2024918 | 23.01 | 6.53 | 14.1 | -0.4261 | snpAH0014 |
LLS70, LLS90 | S3_133697495 - S3_134897269 | 87.81 | 6.74–68.28 | 9.8–87.1 | (-0.980) to 2.080 | snpAH0015, snpAH0016, snpAH0017, snpAH0018, snpAH0020, snpAH0021, snpAH0022, snpAH0024, snpAH0026, snpAH0127, snpAH0128, snpAH0129, snpAH0130, snpAH0131, snpAH0132, snpAH0133, snpAH0134, snpAH0135, snpAH0136, snpAH0137, snpAH0138, snpAH0139, snpAH0140, snpAH0141 |
Rust70 | S2_464902 - S2_768713 | 1.21 | 11.31 | 30.6 | -0.4861 | snpAH0003 |
Rust70, Rust80, Rust90 | S3_133697495 - S3_134897269 | 87.81 | 6.74–71.56 | 15.9–87.1 | 0.12–2.08 | snpAH0149, snpAH0150, snpAH0151, snpAH0152, snpAH0153, snpAH0154, snpAH0155, snpAH0156, snpAH0157, snpAH0158, snpAH0159, snpAH0160 |
High Oleic acid content | ahFAD2A | 65.3 | 7.32 | 8.40 | -8.70 | snpAH0116, snpAH0117 |
High Oleic acid content | ahFAD2B | 90.5 | 13.09 | 25.54 | -9.76 | snpAH0002 |