Figure 4

Subcellular co-localization of BCAT signal peptides fused to GFP with organelle specific markers using transient expression in Nicotiana benthamiana leaves. The HlBCAT1 signal peptide fused to GFP colocalized with the mitochondrial MTRK marker (upper panel) while the HlBCAT2 signal peptide-GFP fusion colocalized with the plastidial PTRK marker (lower panel). Plasmid constructs in Agrobacterium were agro-infiltrated into N. benthamiana leaves and fluorescent protein expression visualized using confocal microscopy. Scale bars represent 20 μm.