The effect of ‘Candidatus Liberibacter asiaticus’ infection on the proteomic profiles and nutritional status of pre-symptomatic and symptomatic grapefruit (Citrus paradisi) plants

Nwugo, Chika C; Lin, Hong; Duan, Yongping; Civerolo, Edwin L

doi:10.1186/1471-2229-13-59

Table 1 Other identified proteins in citrus grapefruit leaves that were down-accumulated in response to Las-infection

From: The effect of ‘Candidatus Liberibacter asiaticus’ infection on the proteomic profiles and nutritional status of pre-symptomatic and symptomatic grapefruit (Citrus paradisi) plants

Spot^a	ASV^b	Protein function/name^c	Accession #^c	Theoretical^d		S^e	M^f	E^g
Spot^a	UP IP US IS	Protein function/name^c	Accession #^c	M _r	pI	S^e	M^f	E^g
		*Energy/Metabolisms*
121		Putative thioredoxin-dependent peroxidase	gi\|119367465	17443	5.15	119	7	70
128		Ascorbate peroxidase 2	gi\|221327589	27724	5.55	162	15	74
161		L-ascorbate peroxidase T, chloroplastic-like isoform 2	gi\|359492510	42300	8.61	223	18	50
188		Coproporphyrinogen III oxidase, putative	gi\|255554717	39324	7.66	162	17	47
189^h		Isoflavone reductase related protein	gi\|3243234	34281	5.92	54	4	19
205		Cinnamoyl-CoA reductase, putative	gi\|255556687	35554	6.16	67	9	39
209		Catalase	gi\|19070130	57669	6.64	110	13	35
		*Regulation/Protein synthesis*
16		31 kDa ribonucleoprotein, chloroplastic	gi\|225456840	38020	4.55	64	8	29
58		Elongation factor Ts	gi\|357500731	82705	4.68	77	11	23
97		Proteasome subunit alpha type, putative	gi\|255538698	29940	5.15	109	10	49
100		Glutamine synthetase plant, putative	gi\|255551511	48172	6.29	183	21	68
105		Glutamine synthetase plant, putative	gi\|255551511	48172	6.29	218	17	59
116		ATP-dependent zinc metalloprotease FTSH 2, chloroplastic-like	gi\|225446693	75921	6.44	197	28	51
136		26S protease regulatory subunit 6b, putative	gi\|255565346	44701	5.49	69	9	30
138		Mitochondrial processing peptidase alpha subunit, putative	gi\|255546263	50379	5.91	163	20	58
149		Nucleoside diphosphate kinase 1	gi\|19570344	16349	5.93	80	7	56
152^h		Transcription factor homolog (Btf3-like) protein	gi\|33945882	17821	5.93	61	3	35
165		Serine-type peptidase	gi\|270342123	46267	8.24	128	8	30
170		S-adenosylmethionine synthetase, putative	gi\|255548295	43620	5.65	217	26	71
178		Nucleoside diphosphate kinase, putative	gi\|255540363	14819	6.92	68	4	35
185		DHAR class glutathione transferase DHAR2	gi\|283135906	23962	6.18	110	8	45
198		Alanine aminotransferase 2 isoform 2	gi\|359495900	54000	6.00	92	13	34
206		mRNA binding protein precursor	gi\|350534514	43638	7.70	97	14	51
207		Alanine aminotransferase 2 isoform 2	gi\|359495900	54000	6.00	188	24	57
		*Chaperones*
10^h		Putative FKBP-type peptidyl-prolyl cis-trans isomerase	gi\|51471872	22889	4.78	46	6	35
30		Heat shock protein, putative	gi\|255555659	73678	5.10	96	13	22
45		Peptidyl-prolyl cis-trans isomerase, putative	gi\|255552604	48362	5.04	99	14	36
57		Chloroplast HSP70	gi\|124245039	76759	5.31	168	22	35
81		Chaperonin-60alpha	gi\|15226314	55491	4.86	210	22	60
86		Heat shock protein 70	gi\|211906496	71346	5.10	118	22	47
111		70 kDa heat shock cognate protein 1	gi\|45331281	71381	5.11	75	9	21
140		Chaperonin-60kD, ch60, putative	gi\|255554262	56809	5.19	79	12	32
		*Unknown*
144		Uncharacterized protein	gi\|225443738	66158	8.50	78	7	20
197		Hypothetical protein VITISV_021486	gi\|147834040	44041	5.98	68	7	24

^a The spot numbers correspond to the numbers given in Figure 2 and Additional file 3: Figure S2.
^b Average spot volume per treatment group; UP, uninfected reference for pre-symptomatic plants; IP, infected pre-symptomatic plants; US, uninfected reference for symptomatic plants; IS, infected symptomatic plants. Average spot volumes separated by letters to show significant difference are presented in Additional file 6: Appendix S1.
^c Protein function/name was determined by http://0-www-ncbi-nlm-nih-gov.brum.beds.ac.uk/BLAST/.
^d Theoretical relative molecular weight (M_r) and isoelectric point (pI) were calculated by http://www.expasy.org/. Observed M_r and pI can be extrapolated from Figure 1.
^e Mascot score of protein hit.
^f Number of matched peptide masses. The sequences of PMF-matched peptides per spot are provided in Additional file 7: Appendix S2.
^g Percent sequence coverage of matched peptides.
^h Protein identification confirmed by MALDI-TOF-MS/MS. Sequencing information is provided in Additional file 7: Appendix S2.
ⁱ Protein identification confirmed by LC-MS/MS.

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ISSN: 1471-2229

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