Table 5 Nuclear Taraxacum kok-saghyz (TK) and T. officinale (TO) species-specific and intraspecific markers
NO.a | Annotation | Forward primer | Reverse primer | Length | Ta | Enzyme | TK | TO |
|---|---|---|---|---|---|---|---|---|
N1 | Tubulin alpha-2 alpha-4 chain | 5′-ATGGTCAGATGCCCAGTGA-3′ | 5′-TGTCGTAGATGGCTTCGTTG-3′ | 540 | 56 | HinfI | Polymorphic | Polymorphic |
N2 | Tubulin alpha-2 alpha-4 chain | 5′-GATTTGGTGAACAATTTGGGTA-3′ | 5′-TCATCATCGGAGATTTCTTTCTC-3′ | 401 | 54 | MspI | Fixed | Fixed |
N3 | Subtilase family protein | 5′-TGGATTTTTATGCACGACACC-3′ | 5′-CCGCACCTTATGCCCTCT-3′ | 358 | 56 | MspI | Fixed | Fixed |
N4 | Tubulin alpha-2 alpha-4 chain | 5′-ATGGTCAGATGCCCAGTGA-3′ | 5′-TGTCGTAGATGGCTTCGTTG-3′ | 540 | 56 | AluI | Polymorphic | Polymorphic |
N5 | Tetraspanin family protein | 5′-AGGGGTCTTGATCTTGGTTG-3′ | 5′-CTTGAGCCATGCGGTAAGTT-3′ | 323 | 54 | DpnII | Fixed | Polymorphic |
N6 | Subtilase family protein | 5′-TGGATTTTTATGCACGACACC-3′ | 5′-CCGCACCTTATGCCCTCT-3′ | 358 | 56 | AluI | Polymorphic | Polymorphic |
N7 | Tetraspanin family protein | 5′-AGGGGTCTTGATCTTGGTTG-3′ | 5′-CTTGAGCCATGCGGTAAGTT-3′ | 323 | 54 | RsaI | Fixed | Polymorphic |
N8 | NAC domain-containing protein 2 | 5′-ATGAGTACCGCCTCGCTAAC-3′ | 5′-GCTTCGCTTTGAACTTCTCC-3′ | 343 | 54 | HinfI | Polymorphic | Polymorphic |
N9 | Aquaporin tip2-2 | 5′-TGGAGATCATCATCACATTTGC-3′ | 5′-GGGTAAATGAGACCAGCTAGACC-3′ | 265 | 56 | BanI | Polymorphic | Polymorphic |
N10 | Aquaporin pip1-1 | 5′-CTCGGAGCCAACAAGTTTTC-3′ | 5′-CAGCGGTGCAGTAGACAAGA-3′ | 295 | 56 | HinfI | Polymorphic | Polymorphic |
N11 | Aquaporin pip1-1 | 5′-CTCGGAGCCAACAAGTTTTC-3′ | 5′-CAGCGGTGCAGTAGACAAGA-3′ | 295 | 56 | MspI | Polymorphic | Fixed |
N12 | Enoyl reductase | 5′-ACTACTCGGAGCGGAAGAGA-3′ | 5′-AATCACCCCAAACCCTAACC-3′ | 606 | 54 | HinfI | Fixed | Polymorphic |
N13 | Enoyl reductase | 5′-ACTACTCGGAGCGGAAGAGA-3′ | 5′-AATCACCCCAAACCCTAACC-3′ | 606 | 54 | MluI | Fixed | Polymorphic |
N14 | Cinnamyl alcohol dehydrogenase 5 | 5′-TGATGTTTACACCGACGGTAA-3′ | 5′-AGCATGAGGAGAGGGGAGAC-3′ | 504 | 54 | HaeIII | Polymorphic | Fixed |
N15 | Cinnamyl alcohol dehydrogenase 5 | 5′-TGATGTTTACACCGACGGTAA-3′ | 5′-AGCATGAGGAGAGGGGAGAC-3′ | 504 | 54 | MluI | Polymorphic | Fixed |
N16 | Cinnamyl alcohol dehydrogenase 5 | 5′-TGATGTTTACACCGACGGTAA-3′ | 5′-AGCATGAGGAGAGGGGAGAC-3′ | 504 | 54 | ScrfI | Polymorphic | Fixed |
N17 | Subtilase family protein | 5′-TGGATTTTTATGCACGACACC-3′ | 5′-CCGCACCTTATGCCCTCT-3′ | 358 | 56 | DpnII | Polymorphic | Polymorphic |
N18 | Subtilase family protein | 5′-TGGATTTTTATGCACGACACC-3′ | 5′-CCGCACCTTATGCCCTCT-3′ | 358 | 56 | BSTEII | Polymorphic | Fixed |
N19 | Subtilase family protein | 5′-TGGATTTTTATGCACGACACC-3′ | 5′-CCGCACCTTATGCCCTCT-3′ | 358 | 56 | Xbal | Polymorphic | Fixed |
N20 | Aquaporin tip2-2 | 5′-TGGAGATCATCATCACATTTGC-3′ | 5′-GGGTAAATGAGACCAGCTAGACC-3′ | 265 | 56 | AluI | Fixed | Polymorphic |
N21 | Aquaporin tip2-2 | 5′-TGGAGATCATCATCACATTTGC-3′ | 5′-GGGTAAATGAGACCAGCTAGACC-3′ | 265 | 56 | ScrfI | Fixed | Polymorphic |