Fig. 2

Autoradiograph of thylakoid membrane fractions after integration of radiolabeled PetD. a PetD in the presence of thylakoid membrane supernatant (S) and membrane pellet (M) after fractionation. b The supernatant (S) and membrane pellet (M) fractions after insertion of PetD and chaotropic agent (NaSCN or NaBr) treatment. c The supernatant (S) and membrane pellet (M) fractions, respectively, after insertion of PetD, but in this case, the translation mixture was solubilised in the presence of 0.2% DDM before insertion into membrane. All experiments were repeated twice and each sample contained 15 μg of total protein that was loaded per well. The MW of radiolabeled product was determined by MALDI-TOF