- Research article
- Open Access
Three members of Medicago truncatula ST family are ubiquitous during development and modulated by nutritional status (MtST1) and dehydration (MtST2 and MtST3)
BMC Plant Biology volume 17, Article number: 117 (2017)
ShooT specific/Specific Tissue (ST) belong to a protein family of unknown function characterized by the DUF2775 domain and produced in specific taxonomic plant families, mainly Fabaceae and Asteraceae, with the Medicago truncatula ST family being the largest. The putative roles proposed for this family are cell elongation, biotic interactions, abiotic stress and N reserve. The aim of this work was to go deeper into the role of three M. truncatula ST proteins, namely ST1, ST2 and ST3. Our starting hypothesis was that each member of the family could perform a specific role, and hence, each ST gene would be subjected to a different type of regulation.
The search for cis-acting regulatory elements (CREs) in silico in pST1, pST2 and pST3 promoters showed prevalence of tissue/organ specific motifs, especially root- and seed-specific ones. Light, hormone, biotic and abiotic related motifs were also present. None of these pSTs showed the same combination of CREs, or presented the same activity pattern. In general, pST activity was associated with the vascular cylinder, mainly in roots. Promoter activation was highly specific and dissimilar during reproductive development. The ST1, ST2 and ST3 transcripts accumulated in most of the organs and developmental stages analysed - decreasing with age - and expression was higher in the roots than in the aerial parts and more abundant in light-grown plants. The effect of the different treatments on transcript accumulation indicated that ST1 behaved differently from ST2 and ST3, mainly in response to several hormones and dehydration treatments (NaCl or mannitol), upon which ST1 transcript levels decreased and ST2 and ST3 levels increased. Finally, the ST1 protein was located in the cell wall whereas ST2 and ST3 were present both in the cytoplasm and in the cell wall.
The ST proteins studied are ubiquitous proteins that could perform distinct/complementary roles in plant biology as they are encoded by differentially regulated genes. Based on these differences we have established two functional groups among the three STs. ST1 would participate in processes affected by nutritional status, while ST2 and ST3 seem to act when plants are challenged with abiotic stresses related to water stress and in physiologically controlled desiccation processes such as the seed maturation.
STs (ShooT specific, as defined for the first time by De Vries et al. , and also referred to as Specific Tissue in the databases) belong to a protein family of unknown function found in specific taxonomic plant families, mainly Fabaceae and Asteraceae, but not in Brassicaceae. Members of this family were first described as cDNA clones in garden pea and chickpea etiolated epicotyls [1,2,3,4], and a comprehensive in silico characterization of the ST family was performed by Albornos et al. .
ST proteins are encoded by multigenic families, where the largest family belongs to Medicago truncatula and is comprised of 6 members. The main feature of STs is the presence of at least one DUF2775 domain whose function is unknown. These proteins have a signal peptide, an N-terminal region with conserved characteristics and several tandems repeats of 25/26 amino acids in variable number . However, ST proteins cannot be associated with any of the previously described members of the category II tandem repeat proteins proposed by Katti et al. . All repeats share a hexapeptide followed by four partially conserved amino acids and a fully conserved tyrosine at position 11 . The repeats are highly conserved within the same ST protein, and the variations among STs are enough to separate them into three groups according to their putative glycosylation pattern (see  for a more detailed description of the proteins).
It is difficult to assign a subcellular location to ST proteins based on in silico predictions even though they contain a signal peptide, indicating their entrance into the secretory pathway, because they lack any other subcellular targeting signals. Recent results from our group point to a dual location of the Cicer arietinum ST1 protein, which is both intra- and extracellular, but the location of other ST proteins still needs to be confirmed .
An expressed sequence tag (EST) profile was determined for the ST family after the analysis of publicly available cDNA libraries in which ST clones were identified . Within the plant, 49% of the sequences came from root and/or radicle libraries, and to a lesser extent from libraries made out of mRNA from seeds (9%), leaves (7%) and stems, epicotyls and hypocotyls (6%). Numerous sequences came from libraries originating from plants grown under biotic interactions (21%) or abiotic stresses (15%). Additionally, the M. truncatula gene expression atlas (MtGEA), also publicly available, confirmed the predominance of transcript accumulation in roots [8, 9].
Several putative roles for ST proteins in plant physiology can be inferred from the information obtained so far in vivo and in silico, which could include these proteins being related to biotic interactions, since their possible involvement in symbiosis has been repeatedly reported [10,11,12,13,14], and to abiotic stress [3, 15]. Also, different members of the ST family have been reported to be related to developmental processes such as early fruit morphogenesis [16,17,18,19], cell elongation [2, 3] or germination , and a putative role as vegetative N storage has also been proposed [2, 7].
To date, several studies regarding the ST family have been carried out in chickpea [3, 5, 7, 15], but although its genome has recently been sequenced  it still remains a species difficult to transform. In contrast, M. truncatula is a well-established model legume with a sequenced genome , and there are numerous laboratory protocols and tools available that facilitate its use. Thus, we decided to study the ST genes in this species, which is also the biggest ST family and comprised of six members. Barrel medic ST genes, namely MtST1 (Medtr4g069810), MtST2 (Medtr3g116440), MtST3 (Medtr3g116430), MtST4 (Medtr3g034640), MtST5 (Medtr3g034610) and MtST6 (Medtr3g107810) encode proteins with a similar structure. MtST1 is located on chromosome 4 and encodes a type I ST, while the other five genes are located on chromosome 3 and encode proteins belonging to type II, as described in Albornos et al. . After taking into account that the EST profile and the MtGEA microarrays showed that MtST transcripts might be differentially distributed throughout the plant, and considering that ST proteins from different species have been associated with diverse functions, we set out to determine if members of the M. truncatula ST family played different or complementary roles in plant physiology, and if their gene expression was differentially regulated.
The aim of this work was to unravel the possible functions of 3 of the 6 ST proteins of M. truncatula, namely MtST1, MtST2 and MtST3 (ST1, ST2 and ST3 in this manuscript), by analysing the promoter sequences of these 3 ST genes in silico, by establishing promoter activity in Arabidopsis thaliana transgenic plants, by studying their transcript accumulation throughout plant development, and in response to different growth conditions, and by determining the subcellular locations of these ST protein.
The ST1 ORF was mis-annotated in the databases
To perform this work, we cloned the promoters and open reading frames (ORFs) of the ST1, ST2 and ST3 genes using the sequences annotated in Phytozome database . The analyses of the annotated sequences confirmed that the gene structure of ST1, ST2 and ST3 was characteristic of the ST gene family  containing introns of 826, 2361 and 693 bp in length, respectively. Furthermore, the analysis of the ST3 gene sequence, as well as its cloned mRNA, indicated that a putative mutation introduces a premature stop codon in the genome sequence, which explains why ST3 C-terminal end is not canonical .
Therefore, the cloned promoters and ORFs of the three genes allowed us to compare their sequences with those found in the databases; only the cloned ST1 ORF showed differences between its sequence and the annotated sequence. After analysing several clones, two differences were confirmed: the first one is the presence of an extra codon for Lys in the annotated ORF, probably because of a wrong in silico interpretation of intron removal, and the second one is a discrepancy with the number of tandem repeats; 11 were present in the database sequence and 13 in the cloned ORF.
The three encoded acidic proteins had the canonical ST protein structure: a signal peptide (21 or 22 aa); a non-repeated sequence (from 43 to 74 aa) and a 26 aa sequence repeated in tandem . ST1, ST2 and ST3 displayed 13, 10 and 14 tandem repeats, respectively, which gave rise to proteins of different length and molecular masses. ST1 and ST3 had 430 aa with a calculated MW of 48.6 kDa and ST2 had 359 aa and a MW of 40.7 kDa.
Several cis-acting regulatory elements were present in ST1, ST2 and ST3 promoter sequences
In silico analysis of promoters has become an important source of information to study the transcriptional regulation of genes. Core promoters can be TATA or TATA-less depending on the elements they have to initiate transcription. The TSSP software predicted the transcription start site (TSS) (−58, −47 and −69 for pST1, pST2 and pST3, respectively), and the presence of a TATA box (−92, −81 and −102 for pST1, pST2 and pST3, respectively) in the three promoters studied, with a linear discriminant function over 0.02 that validates the predictions.
An ever-increasing number of cis-acting regulatory elements (CREs) are being collected in the databases. However, their presence within a given promoter of interest and their association with a putative activation profile requires in vivo analysis.
Examination of the first 1000 bp in ST promoters (pSTs) using the PLACE and PlantCARE databases identified 113 different CREs (614 motifs). pST1 had the least number of motifs (166) and pST2 and pST3 had 225 and 223, respectively (Additional file 1). The CREs were classified into 7 categories, according to the functional information provided by the databases (Fig. 1a). These categories were: 1) CREs controlling specific activities in organs or tissues; 2) related to light regulation; 3) hormone response; 4) establishment of biotic interactions; 5) abiotic stress response; 6) enhancers; and 7) unclassified (other) (Additional files 1 and 2). Each of the three pSTs had its own specific combination of CREs (Fig. 1b, c; Additional file 2).
The elements within the tissue/organ specific category, which represented at least 44% of the motifs found in each of the pSTs, were those associated with the meristem, root, mesophyll, flower and seed subcategories, and were present in all three pSTs. In contrast, the CREs found in xylem were only present in pST2, CREs in guard cells were only present in pST1 and pST3, and CREs in fruit were only present in pST1 (Fig. 1b; Additional file 1). The most widespread motif in the meristem subcategory, NTBBF1ARROLB, which is associated with apical meristems and specifically with vascular tissues and auxin induction, was absent in pST2 (Fig. 1b; Additional file 2). The CREs found in the root subcategory were especially frequent in pST2 (9%). However, the ROOTMOTIFTAPOX1 element, specific to the roots of adult plants, was the only CRE found in all of the pSTs, and was the most abundant in pST1 and pST2. RAV1AAT, related to gene expression in rosette leaves, was most abundant in pST3 (Additional file 2).
Known CREs associated with reproductive development were also found in the pSTs. Flower-specific elements were well represented (6–8%) mostly those associated with pollen expression (Additional file 2). The motifs GTGANTG10 and POLLEN1LELAT52 were found in all three pSTs however, POLLEN2LELAT52, the co-dependent element of POLLEN1LELAT52, was only present in pST1. The high number of seed-specific CREs is also noteworthy, being more abundant in pST1 (27%) than in pST2 and pST3 (21% and 22%, respectively). Seed motifs were divided into monocot or dicot subcategories, depending on their origin (Additional file 1). pST1 and pST3 presented nearly the same number of motifs of both subcategories, while pST2 had fewer of the elements found in monocot seeds (8% vs. 13%). A total of 20 different seed-related CREs, 11 of them found in the promoters of genes encoding seed storage proteins, was also present in pST1, pST2 and pST3 (Fig. 1b; Additional file 2).
Numerous light-associated CREs (ranging from 16 to 22%) were found in the pSTs (Additional file 1). Three different subcategories were considered: light-regulated elements (LRE), with 22 motifs in the three pSTs; circadian elements (1 motif found in pST3); and phytochrome elements (2 motifs absent in pST3) (Fig. 1b; Additional file 1). Among the LREs, the most represented motifs were the 4 bp GATABOX (32 times), conserved in the promoters of genes encoding chlorophyll a/b binding proteins, and GT1CONSENSUS (20 times), a GT-1 binding site mainly related to light regulation, prevalent in pST2 (Additional file 2). These CREs, together with IBOX and ABRELATERD, were the only ones found in all of the pSTs (Additional file 2). Apart from ABRELATERD, another two motifs associated with gene expression in darkness, REALPHALGLHCB21 and SORLREP3AT, were also found (Fig. 1b; Additional file 2).
The category of CREs involved in the hormonal regulation of gene expression represented approximately 10% of the elements found in pST2 and pST3, but only 6% in pST1 (Additional file 1). CK elements, mainly ARR1AT, were the most abundant and the only ones found in every pST, reaching 7% in pST2 (Additional file 1). ABA motifs, such as ABRERATCAL, appeared in pST2 and pST3 but did not appear in pST1. The unique IAA element was exclusively found in pST1, while GA elements were absent in pST2. Promoters containing ET- and SA-related CREs (pST1 and pST2) lacked JA specific elements (pST3) and vice versa (Fig. 1c; Additional files 1 and 2).
The pSTs also contained elements related to biotic interactions, including symbiosis and pathogenesis, that ranged from 9% (pST1 and pST2) to 11% (pST3) (Additional file 1). There were two motifs related to symbiosis known to drive gene expression in the infected cells of root nodules in different species including M. truncatula: OSE1ROOTNODULE, which was absent in pST3, and OSE2ROOTNODULE present in all three pSTs (Fig. 1c; Additional file 1). The pathogenesis subcategory contained 9 different CREs that appeared 39 times overall (Additional file 1). The most represented were BIHD1OS (13 matches), associated with disease, and GT1GMSCAM4 (8 matches), related to pathogen attack and salinity (Fig. 1c; Additional file 2). The presence of 4 different W-box binding motifs of the WRKY transcription factors (TF) (14 matches) is also worth noting (Additional file 2).
Elements associated with abiotic stress represented 10%, 11% and 12% of the motifs in pST1, pST2 and pST3, respectively (Additional file 1). Within this category we established five subcategories. A small number of CREs related to anoxia (absent in pST3) and heat shock (absent in pST1) were found. All three pSTs contained elements associated with dehydration, low temperature and nutrition subcategories. Dehydration motifs, including water stress and drought tolerance, were the most abundant CREs (Additional file 1). Six of the 9 drought-response elements were MYB transcription factor binding sites (20 matches altogether) and 1 was the MYC transcription factor binding site MYCCONSESUSAT, the most represented element in the abiotic category (17 matches) (Fig. 1c; Additional file 2). In the nutrition subcategory, the sulphur deprivation element (SURECOREATSULTR11) was found in all three pSTs, while the ammonium-response (AMMORESIIUDCRNIA1) and phosphate-starvation (P1BS) elements were unique to pST1. In contrast, iron starvation (IRO2OS) and copper responsive elements (CURECORECR), present in pST2 and pST3, were absent in pST1 (Additional file 2).
Within the enhancer regions category, we distinguished between general enhancers, such as 2 TA-rich enhancer motifs in pST3, and specific enhancers that increase transcription in specific genes or tissues, such as the Skn-1 motif, related to endosperm, in pST1 and pST2 (Additional files 1 and 2). Finally, in the category of others, we included elements involved in cell cycle and gene expression in plastids, which represented less than 2% of CREs found in all three pSTs (Additional files 1 and 2).
Activity of pST1, pST2 and pST3 in Arabidopsis thaliana was high in the root vascular cylinder and showed maximum differences during reproductive development
Arabidopsis transgenic plants with a ST gene promoter driving the expression of the GUS reporter gene (pST::GUS) were generated to determine the activity of these promoters. The wild-type plants used as controls did not show GUS activity; therefore, no images have been included. Two pST3 constructs of different lengths were made: pST3·F1 refers to the longest construct consisting of 2390 bp, and pST3·F2 to the shortest one with 1073 bp. Both constructs displayed the same activity pattern, with pST3·F1 activity higher than pST3·F2 in vegetative organs. All of the images shown in Figs. 2, 3, and 4 came from the pST3·F1::GUS transgenic plants.
The analysis of the activity of the pSTs was carried out in 3- (Fig. 2a, b) and 10-d-old (Fig. 2c, d) dark-grown (etiolated) (Fig. 2a, c) and light-grown (green) (Fig. 2b, d) seedlings. In general, GUS activity was found mainly in the cotyledons and root vascular cylinder, and activity in the cotyledons was higher in the etiolated and youngest plants (Fig. 2). All three promoters showed activity in the cotyledons of 3-d-old etiolated and green seedlings (Fig. 2a, b); activity was the faintest in the green seedlings (Fig. 2b). At 3-d-old, pST2 and pST3 were also active in roots, both in the apex and in the vascular cylinder, although at different intensities (Fig. 2a2, a3, b2, b3). In dark-grown seedlings pST3, but not pST2, was also active at the hook level (Fig. 2a3). The activity of pST2 and pST3 observed in the shoot apex and in the root-hypocotyl junction in light-grown seedlings (Fig. 2b2, b3) was quite intense.
Almost no activity, except for pST3, was found in the cotyledons of 10-d-old etiolated seedlings (Fig. 2c). In these plants, only pST2 remained slightly active in the roots, and its activity extended towards the hypocotyl vascular cylinder (Fig. 2c2). The three promoters were active in 10-d-old light-grown pST::GUS plantlets (Fig. 2d), and their activation pattern was maintained in the vascular cylinder, either in leaves, hypocotyl and/or radicles, as vegetative growth progressed (Fig. 3) regardless of the plants’ age. In most cases, the roots (Fig. 2d, 3a) were more stained than the above ground tissues (Fig. 3b–d), where blue staining was observed mainly in the leaf veins and hypocotyl. However, differences were observed in the activity pattern of each ST promoter.
The analysis of the promoter activities in roots from 10 to 24-d-old transgenic plants (Fig. 3a) showed that pST1 and pST3 were inactive in the root apex (Fig. 3a1, a3), unlike pST2 that was active both in primary and secondary root apical areas (Fig. 3a2). Although the activity of pST1 was discontinuous, blue staining was mainly located in the vascular cylinder, especially where the econdary roots emerged (Fig. 3a1). The most active promoter in roots was pST2, showing blue staining throughout the central cylinder, both in primary and secondary roots. The darkest staining was observed in the insertion zone of secondary roots, where activity was initiated as soon as a root started to develop (Fig. 3a2). By contrast, pST3::GUS plants showed higher GUS activity in the basal area of their primary root, being fainter or absent in the rest of the root and with no activity in the secondary roots (Fig. 3a3, c3).
Regarding the aerial organs, pST2 and pST3 were not very active, and the staining was restricted to hydathodes in the leaves and veins of the cotyledons (Fig. 3b2, b3, d3), being almost undetectable in the pST1::GUS transformants (Fig. 3b1, c1). pST2::GUS and pST3::GUS plants showed blue colour in the lamina of the youngest leaves, mainly in the proximal part of the organ, (Fig. 3b2, b3) but only pST3 was active in the vascular bundle of leaf petioles (Fig. 3b3, c3). Also, two different pST activity patterns were observed (Fig. 3c) in the hypocotyl. While pST1 was active only in some individual cells in the epidermis and vascular cylinder (Fig. 3c1), pST2 and pST3 showed a highly continuous GUS activity, but only in the central cylinder (Fig. 3c2, c3). pST2 and pST3 were active in the centre of the rosette (Fig. 3c2, c3, d3), most probably where new leaves start to develop or when the flower stem starts to emerge. In fact, both promoters were active in the flowering stem, although with different patterns; pST2 acted in the veins (Fig. 3c2) and, once developed, in guard cells (Fig. 3d2 and detail in the square). pST3 was seen throughout the stem, but it was difficult to determine its precise activity (Fig. 3d3).
The activity of the three pSTs differed greatly during flower and silique formation (Fig. 4), confirming their specificity during reproductive development. Fig. 4 shows the structures with GUS activity sorted according to their developmental stage from the beginning of flower formation to the mature silique.
The activity of pST1 along flower development was restricted to the androecium (Fig. 4, a1-d1). High pST1 activity was detected in 4 specific points at the base of flower bud, coinciding with the origin of the four long stamens (medial) (Fig. 4a1, b1). pST1 was active during pollen formation, from medium stages to maturity (Fig. 4c1, d1). In fact, the pollen of long stamens stained an intense colour of blue earlier than the pollen from the short stamens (lateral) (Fig. 4c1). During seed and fruit development, the GUS activity driven by pST1, was only observed in the joint between funiculus and seed at early/medium stages of fruit development (Fig. 4e1 and detail).
pST2 was style specific in the beginning of flower growth, and this activity continued along gynoecium development (Fig. 4a2-c2). However, later on in development, blue staining was also observed in the stamen filament, close to the anthers and all along its vascular tissue (Fig. 4a2, b2, c2). A faint GUS activity was also observed in petal and pedicel vasculature, as well as in the pedicel insertion point (Fig. 4a2, b2, c2). The activity of pST2 was transiently up regulated in the chalazal endosperm tissue at a specific seed developmental stage (Fig. 4d2).
Unlike pST1 and pST2, pST3 was related mainly to female gametophyte development. In addition, pST3 was active at the base of stamen filaments along their development (Fig. 4a3, b3), in several pollen grains (Fig. 4a3), and in the style at initial flower growth (Fig. 4b3). It was also possible to detect blue staining in the female gametophyte before fertilization (Fig. 4b3), and the activity of the promoter was maintained but decreased throughout seed development (Fig. 4b3-f3). At the beginning of seed development dark blue staining was observed in the whole seed, within the endosperm and embryo (Fig. 4d3). Later, the activity decreased and finally was restricted to the chalazal endosperm, the same that was observed for pST2 (Fig. 4d3, e3). Finally, faint GUS activity remained in embryonic cotyledons of pST3::GUS plants (Fig. 4f3, g3).
ST1, ST2 and ST3 transcript accumulation was ubiquitous during plant development and specific under different growth conditions
Analysis of M. truncatula ST1, ST2 and ST3 transcript accumulation during development was performed in seeds 24 h post imbibition (hpi) throughout the development of dark- and light-grown seedlings and in adult green plants (Fig. 5). Photographs of M. truncatula at the stages studied are showed in Additional file 3 and the agarose gel electrophoresis of sqRT-PCR amplicons in Additional file 4. Only those changes statistically significant will be remarked in this section.
All three transcripts were detected in seeds 24 hpi (Fig. 5a). In 3-, 6- and 10-d-old dark-grown seedlings ST1, ST2 and ST3 transcripts were detected both in aerial organs (namely hypocotyl and cotyledon) and in roots, except for ST1 transcripts that were not found in the aerial part of 10-d-old etiolated seedlings (Fig. 5a). The amount of transcripts was, in general, higher in roots than in the aerial parts of the plant. The maximum transcript levels were detected in 3-d-old radicles and decreased with age although at different rates: either progressively (ST1), abruptly (ST2) or delayed (ST3), with the transcript levels in 6-d-old seedlings being as high as those in 3-d-old seedlings (Fig. 5a).
A higher level of ST transcript accumulation was observed in light-grown plants (Fig. 5b) as compared to the dark-grown plants. Maximum transcript levels were also found in the roots of 3-d-old seedlings, being more noticeable for ST1 and ST2, while the levels of ST3 also remained high in the other organs/stages analysed (Fig. 5b). Differences in transcript accumulation, when comparing root and aerial parts, were found among the STs. ST1 accumulation was very similar between both parts except in 3-d-old seedlings; ST2 was clearly higher in roots in the time lapse analysed and ST3 displayed similar levels in roots and aerial parts (Fig. 5b).
Accumulation of transcripts in the roots, leaves, flowers and pods of mature plants, as well as in the late filling stage of seeds (green seed), were also investigated (Fig. 5c and amplification 5c1). Transcript levels at 3 different stages of leaf, flower and pod development were compared (1 to 3 from youngest to oldest; Additional file 3). All transcripts, except ST3, were much more abundant in roots than in leaves, where they were barely detected (Fig. 5c1). ST3 accumulated at a high level in small developing leaves (stage 1) decreasing dramatically afterwards (Fig. 5c).
ST1, ST2 and ST3 transcripts were detected throughout all the reproductive stages analysed with a similar pattern (Fig. 5c1). Along flower development small variations, not statistically significant, were observed (Fig. 5c1). However, along pod development the three ST transcripts especially accumulated in pod stage 3 and in green seeds (Fig. 5c).
In addition to the study of transcript levels throughout plant development, ST accumulation in 7-d-old seedlings exposed to different treatments was analysed (Fig. 6). We grouped the treatments taking into account the putative roles proposed for the ST proteins thus we considered those treatments mainly related to normal growth and development (Fig. 6a), ABA and abiotic stress situations (Fig. 6b) except different temperature conditions (Fig. 6c), and those whose participation in biotic interactions has been thoroughly established (Fig. 6d). Obviously, this is a broad classification owing to the complex interrelations between hormones and physiological processes (i.e. the role of strigolactones both in branching and in biotic interactions), but it helps to clarify and analyse the obtained results.
As STs have been associated with elongation , we analysed the effect of hormones mainly related to normal growth and development in ST transcript accumulation (Fig. 6a). The results indicated that ST1 behaved differently than ST2 and ST3, which shared the same profile of mRNA levels. ST1 transcripts were the only ones that significantly increased after IAA, CK and SL treatments, but were reduced by treatment with GA (Fig. 6a). ST2 and ST3 transcripts showed slight changes, such as a reduction in RNA levels by IAA, GA and BL and an increase in transcript accumulation by CK and SL, but none of these changes were statistically significant (Fig. 6a).
The second group of conditions tested was related to abiotic stress and included the study of: ABA; salt (NaCl) and osmotic (mannitol) stresses; nutrient starvation (N and Pi) (Fig. 6b); and exposure to unfavourable growth temperatures (4 °C and 37 °C) (Fig. 6c). When plants were grown in medium containing ABA, NaCl or mannitol, ST1 transcript levels decreased dramatically, while ST2 and ST3 increased significantly (Fig. 6b). Nutritional starvation only clearly increased ST1 accumulation (Fig. 6b). Finally, high temperatures had a significant inhibitory effect on ST transcript accumulation, while low temperatures had almost no effect (Fig. 6c).
To complete this study, the effect caused on ST transcript accumulation by hormones involved in responses to biotic stress (ET, MeJA and SA) and mechanical wounding (Fig. 6d) was analysed. These treatments produced statistically significant changes in the levels of all three ST transcripts, where ST3 was the least affected. Generally, the application of ET and SA decreased ST transcript accumulation, except ET for ST3, while MeJA induced their levels (8 times for ST1 and 2 times for ST2 and ST3) (Fig. 6d). The effect of MeJA was slightly reduced when applied together with ET or with GA, and drastically reduced if a mixture of MeJA and SA was added to the growing trays (Fig. 6d). All of these results were in agreement with the individual effect of each hormone. Furthermore, mechanical wounding only significantly increased the transcript levels of ST1, although, not as intensely as the MeJA treatment.
ST1 protein was located in the cell wall while ST2 and ST3 had a double location
The cellular compartment where a protein is located determines the range of functions that it may perform. Thus, ST proteins fused to green fluorescent protein (GFP) at its C-terminal end were localized by confocal microscopy within root cells of transgenic A. thaliana (p35S::ST-GFP) (Fig. 7).
The results obtained when the roots were mounted in water (turgid cells) showed that the three ST proteins were localized at the cell boundaries and green fluorescence co-localized with red fluorescence from propidium iodide (PI) stained cell wall (Fig. 7). However, turgid cells made it difficult to distinguish whether the fusion ST-GFP proteins were clearly in the wall. To avoid this problem, roots were mounted in mannitol to cause cell plasmolysis and to separate the plasma membrane from the cell wall. In this case, ST1-GFP clearly co-localized with PI stained cell wall (Fig. 7). However, ST2 and ST3 were detected in the cell wall and also in the contracted cytoplasm, as deduced by the presence of green fluorescence inside the cell that did not co-localize with red fluorescence. This dual location was clearer in p35S::ST3-GFP plants (Fig. 7).
ST proteins were first described in pea [1, 2, 4] and subsequently characterized in chickpea . The roles of these proteins, containing tandem repeats [2,3,4,5] and associated with the DUF2775 domain , remain elusive. M. truncatula has the largest ST gene family (6 ST genes) with a defined genome distribution, suggesting that some MtST genes may have arisen by duplication and then later became subfunctionalized or neofunctionalized (reviewed in ). The starting hypothesis of our studies regarding the function of STs of M. truncatula was that each family member might have a specific role, and hence, that each ST gene might be subjected to a different type of regulation throughout plant development and/or in response to environmental factors. Accordingly, we have studied the regulation of the M. truncatula ST family by analysing their promoter regions (both in vivo and in silico), their transcript accumulation and the subcellular location of the proteins. The results obtained have enabled us to establish three functional groups among the whole family (data not shown). In this work we present two of them that include three members of the family, namely ST1, ST2 and ST3. ST1 protein is a type I ST, whose gene is located in chromosome 4, and ST2 and ST3 are type II STs, encoded by two consecutive genes in chromosome 3 .
The regulation of gene expression at the promoter level is mainly controlled by type, number, position and combination of CREs localized in the proximal zone, mainly in the first 500 bp upstream the TSS . Furthermore, post-transcriptional regulation determines the accumulation of mRNA, and to some degree the amount of protein available within the cell. The activity pattern of several plant promoters is conserved even between angiosperm and gymnosperm , indicating the universal functionality of CREs, although differential performance has also been reported [27, 28]. Taking advantage of the conserved functionality of CREs among species, we have studied the ST gene promoters of M. truncatula using the model plant Arabidopsis thaliana.
Bioinformatics indicates that pST1, pST2 and pST3 have a TATA box determining the TSS. Although it is more common to find promoters without a TATA box in plant genes , its presence is not unusual in duplicated genes , as could be the case of M. truncatula ST family. The three pSTs analysed contain CREs that respond to endogenous factors (organ or tissue specific, hormones and enhancers) and environmental factors (light, abiotic stress and biotic interactions) (113 different motifs), although they differ in the combination and number of the different CREs (Fig. 1, Additional files 1 and 2). The amount of CREs found in each pST was very high (from 166 to 225 in the 1000 bp analysed) (Additional files 1 and 2) as often happens in in silico promoter analysis, given the small size of CREs (as an example a 4 nt CRE could appear once in 256 bp by chance). Consequently, only a small percentage of the CREs found in silico (around 5%) would constitute real binding sites for TFs and be functional . Therefore, in this work, we will only discuss such CREs related to the in vivo promoter activity and the transcript accumulation analysed. The ST gene expression profiles coming from the microarray experiments and available at MtGEA were also considered [8, 9]. Although this information is of high value for the study of most transcripts, it should be carefully considered with respect to the highly conserved tandem repeat proteins, such as STs.
According to our results ST1, ST2 and ST3 profiles share several characteristics, mostly during vegetative development. Concerning pST activity, we are able to highlight several common features such as activity both in light and darkness, highest activity in youngest seedlings, predominant activity in roots, and activity associated with the vascular cylinder and leaf veins (Figs. 2, 3). The transcript accumulation pattern (Fig. 5) usually confirms the promoter activity profile (Figs. 2, 3) and allows us to contextualize our results. ST1, ST2 and ST3 transcripts are ubiquitously detected in almost all of the developmental stages analysed (Fig. 5), which could suggest that these ST proteins carry out their function throughout plant development.
M. truncatula pST1, pST2 and pST3 are active (Fig. 2) and transcripts accumulate (Fig. 5a, b) in seedlings growing in darkness and in light, in agreement with previous reports in pea and chickpea [1, 3]. However, while promoter activities are generally higher in etiolated seedlings, primarily in cotyledons (Fig. 2a), transcript levels show the contrary (Fig. 5a, b), which is opposite to ST transcript accumulation described in pea . CREs associated with the control of gene expression by light are abundant in all pSTs. On the one hand, the presence of elements driving transcription in darkness, ABRELATERD CRE (ABRE-like element required for expression of erd1 in dark-induced senescence) mainly in pST3 (6 times), SORLREP3AT (motif in light-repressed promoters) (1 in pST3), and REALPHALGLHCB21 (associated to an increase of expression in darkness) in pST1 and pST2 (Fig. 1b; Additional file 2), could explain the promoter activity and the mRNA accumulation in etiolated plants (Figs. 2, 5a). Additionally, typical LREs (as defined by Terzaghi and Cashmore ) are also present in pST, however no individual LRE can confer light-responsiveness on its own; it is their combination that ultimately determines the effect [28, 32]. The activity of all promoters in green adult plants could be explained by the presence of the typical LRE GT1CONSENSUS motif that confers light induction in mature plants, but not in seedlings .
Besides its usefulness in studying light responses, comparing green and etiolated seedlings is a good way to establish the relationship of a protein with elongation, one of the functions postulated for STs in chickpea [3, 7, 15, 20]. Here, the decrease of pST activity (Fig. 2a, c) and ST transcript accumulation (Fig. 5a, b) along plant growth, lead us to consider that barrel medic ST proteins are not related to elongation. Although certain data regarding ST1 (the increase in ST1 transcript accumulation by IAA, CK and SL [Fig. 6a]), could suggest the involvement of ST1 in elongation, other data (the low transcript accumulation in etiolated seedlings with a high elongation rate [Fig. 5a], the decrease after GA application [Fig. 6a], or the strong induction of transcript accumulation by MeJA, a negative growth regulator [Fig. 6d] [33, 34]), cause us discard this function for ST1 as well.
Throughout plant development, higher pST activity and transcript accumulation in the earlier stages of vegetative organs (Fig. 2, 5), as well as in apical areas - mainly in pST2 and pST3 (Figs. 2, 3) -, is consistent with the presence of CREs associated with specific expression in meristematic zones such as NTBBF1ARROLB (Fig. 1b, Additional files 1 and 2). This CRE is also a vasculature-specific element (Additional file 2), which explains the other common features of pST activity, such as the strong association with vasculature in both aerial parts of the plant and in roots (Figs. 2, 3). Interestingly, CREs related to xylem are only found in pST2 (Fig. 1b; Additional files 1 and 2).
ST1, ST2 and ST3 transcripts levels are, in general, higher in roots than in aerial parts (Fig. 5), similar to what occurs in chickpea radicles  and grapevine roots , but not in garden pea . These results were also suggested from the EST profile  and MtGEA database [8, 9], and agree with the predicted percentages of root-related CREs (Fig. 1a, Additional file 1) and the activity of pSTs in roots (Figs. 2, 3). In fact, the high level of pST2 activity in roots (Fig. 2b2, 3a) corresponds to the high number of root motifs present (9%), mainly ROOTMOTIFTAPOX1 (Fig. 1a; Additional files 1 and 2), allowing gene expression in the roots of adult plants (Additional file 2). In contrast to previous studies in C. arietinum [3, 20] and P. sativum [1, 2, 4], which showed a high transcript level of STs in etiolated epicotyls and stem internodes, no CREs related to stem, hypocotyl and/or epicotyl are found (Additional file 2), and no general promoter activity is detected in such etiolated organs (Fig. 2a).
Apart from the common features described above, our results showed fundamental differences among STs that can be divided into two main categories: reproductive development (Fig. 4) and responses to different treatments (Fig. 6). The putative function of STs during flower, fruit and seed development remains controversial depending on the species [3, 4, 16]. Barrel medic pST activity showed a highly specific pattern (Fig. 4), and few CREs associated with the reproductive stage have been found (Fig. 1a, b; Additional files 1 and 2). Based on these differences we decided to establish 2 functional groups: the first one includes ST1 and the second one includes ST2 and ST3.
ST1 is the only gene studied whose promoter carries both co-dependent CREs related to late pollen-specific expression (POLLEN1LELAT52 and POLLEN2LELAT52), as well as GTGANTG10 (9 times) (Fig. 1b, Additional file 2), which correlates with in vivo pST1 activity (Fig. 4) and ST1 transcript accumulation in flower stages 1 and 2 (Fig. 5c1). During male gametophyte development there are genes that are expressed both early (undetectable in mature pollen) and late (accumulate as pollen matures) . Therefore, the blue staining, driven by pST1, points to a late pollen-regulated expression (Fig. 4c1, d1), suggesting that the ST1 protein could accumulate in the male gametophyte, although, its putative role remains elusive.
The M. truncatula ST1 protein is very similar to C. arietinum ST1 (CarST1) as both are type I ST proteins, as defined by Albornos et al. . Regardless of the number of ST proteins that a given species may have, there is always only one type I ST, which suggests the same conserved function for all of them. Since Albornos et al.  provide evidence describing CarST1 as a type of vegetative storage protein related to N storage and mobilization in both vegetative organs and in seeds, we also discuss this possibility for ST1 in M. truncatula. The fact that ST1 transcript levels increase during N starvation (Fig. 6b), when N reserves should be metabolized, and the fact that etiolated cotyledons show pST1 activity (Fig. 2), when storage proteins in cotyledons should be degraded to give N and carbon , are in disagreement with a role as reserve protein for barrel medic ST1. This is contrary to what has been described in chickpea, where no CarST1 transcripts are found in etiolated cotyledons  and where protein levels decrease as germination proceeds . These differences could be due to the different fates of the cotyledons of chickpea (hypogeal germination), Arabidopsis or barrel medic (epigeal germination), where in chickpea the cotyledons degenerate and in Arabidopsis or barrel medic the cotyledons behave as leaves. Also, it should be considered that ST1, unlike CarST1 , is clearly located only in the apoplast (Fig. 7) and not in protein bodies inside cells, the place where several reserve proteins accumulate .
However, although ST1 does not seem to be a reserve protein, we cannot discard that pST1 may be regulated by the nutritional status of the plant, as pST1 is the only one that contains the CRE AMMORESIIUDUNIA (Fig. 1c, Additional file 2), associated with genes involved in N metabolism, and also the CRE P1BS (Fig. 1c, Additional file 2), related to transcriptional activation of phosphate transporters induced in mycorrhizal symbiosis . Different data support this hypothesis: ST1 transcripts accumulation increases under N and Pi starvation (Fig. 6b); a high number of ST1 ESTs were found in cDNA libraries made from plants establishing symbiosis with N fixing bacteria and arbuscular mycorrhizal fungi or under Pi starvation ; and three independent microarray experiments available at MtGEA (including ) indicate that ST1 mRNA increases under N limiting conditions. Also supporting this idea, barrel medic ST1 transcripts were strongly induced by MeJA (Fig. 6d), which has a major role in plant defence and alters N transport and distribution [40,41,42] since pathogen attack increases the sink force of an organ (reviewed in ). Finally, the pST1 activity in funiculi (Fig. 4e1) supports its function in maternal and/or embryo nutrient transport and assimilation, as the funiculus provides a way for nutrients to move from the mother plant [44, 45].
In the second functional group, promoter activity and transcript accumulation point to a broad presence of ST2 and ST3 in the plant regardless of age, where they are more represented than ST1 in almost all conditions studied (Fig. 2-5). This result agrees with previous EST profile results that indicate that only ST2 and ST3 were detected in plants growing under normal conditions . ST2 and ST3 show similar mRNA accumulation profiles, with high transcript accumulation in pods as well as in green seeds (Fig. 5c). This is in accordance with the fact that both pST2 and pST3 are active in seeds (Fig. 4d2, c3-g3), and with the presence of several seed-specific CREs within their sequences (Fig. 1a, b; Additional files 1 and 2), some of them found in promoters of genes encoding seed storage proteins (SSP). However, it has been checked that none of the combinations of CRE that usually drive the expression of SSP, such as B-box and RY-G-box in dicotyledonous (reviewed in ), are present either in pST2 or in pST3. Moreover, there are other characteristics that allow us to discard that ST2 and ST3 are SSP, i.e., pST2 and pST3 promoters are active in developmental phases other than seed filling (Figs. 2, 3, 4) and transcripts accumulate all through plant development (Fig. 5).
Once the role of ST2 and ST3 as SSP has been discarded, the high level of ST2 and ST3 transcripts found in green seeds in the late filling phase, when storage accumulation still takes place and the water content diminishes by half and the desiccation tolerance increases exponentially , might relate these proteins with a physiological water deficit. Moreover, ST2 and ST3 transcripts are strongly induced by ABA, NaCl and mannitol (Fig. 6b), what lead us to consider a role in situations of physiological and/or environmental water deficit, resembling late embryogenesis abundant (LEA) proteins (reviewed by ). ST2 and ST3 amino acid sequence share characteristics with LEA proteins such as high hydrophobicity, high content of Gly and small amino acids like Ser or Ala, a high proportion of charged amino acids and the lack of Cys and Trp (reviewed by ). Furthermore, several LEA proteins have tandem repeats and could be intrinsically unstructured proteins [49,50,51], as has been pointed out for ST2 and ST3 . The promoter activity pattern (Figs. 2, 3) and the transcript accumulation profile (Figs. 5, 6) indicate that ST2 and ST3 proteins could show a similar distribution as some of the LEA proteins, not only under dehydration conditions but also during plant normal growth [52,53,54]. Thus, the accumulation of ST3 transcripts in leaves at early developmental stages (Fig. 5c1) as occurs for several LEA proteins , the pST2 activation in apical root (Fig. 2a2, b3, 3a2) where numerous LEA proteins accumulate , the presence of both STs and LEA in mature organs such as roots and flower , and finally, the accumulation of LEA proteins in pollen grains  where pST3 transiently activates (Fig. 4a3), support this notion. Also, pST3 has a CRE motif associated with ABA-regulated late embryogenesis expression (DRE2COREZMRAB17; Additional file 2).
All of these similarities between ST2 and ST3 and LEA proteins suggest that ST2 and ST3 could play a role in response to water deficit. Intracellular accumulation of ST2 and ST3 is compatible with this function. In fact, the subcellular location of ST2 and, mainly ST3, can contribute to membrane stability when the cell suffers an osmotic shock (Fig. 7). This role in dehydration is supported by the presence of specific CREs in their promoters. TFs that modulate gene expression in situations of abiotic stress bind to ABA responsive elements (ABRE) in ABA-dependent responses. In this sense, pST2 and pST3 contain 3 and 4 ABRE sequences, respectively (ABRERATCAL, Fig. 1c; Additional files 1 and 2). They also contain G-boxes (CACGTGMOTIF; Additional file 2), which are coupling elements necessary for induction of gene expression in response to ABA and other stimulus [56,57,58], and MYB and MYC TF binding sites (13 and 15 motifs, respectively), that coordinate regulation of gene expression in abiotic stress (reviewed in [59, 60]). W-box elements, binding site of WRKY TF, were also found in pST2 and pST3 (Additional file 2). Although traditionally associated to biotic stress, WRKY TF have also a relevant role in coordinating responses to abiotic stress [61,62,63]. The induction of WRKY TF mediated by MeJA in response to water deficit has been determined , where the increase of ST2 and ST3 accumulation in plants treated with this hormone (Fig. 6d) is noteworthy.
A previous analysis of the EST profile  also supports the role of ST2 and ST3 in response to dehydration and other abiotic stresses, since their transcripts accumulate in situations of drought and salinity, as was found in the microarray assays collected in MtGEA . Also, transcriptomic analysis in Trifolium repens shows an increase in ST transcripts when drought-tolerant plants grow under water stress . By contrast, water stress decreases ST transcript accumulation in chickpea seedlings [3, 15] as occurs in M. truncatula ST1 (Fig. 6b). This confirms that the various STs behave differently under abiotic stress.
The ST proteins studied in this work, ST1, ST2 and ST3, are ubiquitous proteins that could perform different/complementary roles in plant biology as they are encoded by genes differentially regulated. The analysed pSTs show different combinations of specific CREs, and they also present different activity patterns. However, all pSTs were rich in tissue/organ specific CREs with the highest number representing those specific to roots and seeds. Common features in promoter activity and also in transcript accumulation have been found, mainly during vegetative development, such as the abundance in roots and in the vascular cylinder. Nevertheless, significant differences, mainly during reproductive development and in response to different stimuli, led us to consider two functional groups, which confirms our initial hypothesis. ST1 may participate in processes affected by nutritional status, while ST2 and ST3 seem to act when plants are challenged with abiotic stresses related to water stress and in physiologically controlled desiccation processes such as the seed maturation.
Plant material and growth conditions
Arabidopsis thaliana ecotype Columbia-0 (Col-0) was used as the host for transformations. Seeds were sterilized, stratified and germinated in Petri dishes on one-half-strength Murashige and Skoog (MS)  agar medium and 1% (w/v) sucrose, as described in Albornos et al. .
These seedlings were maintained in Petri dishes up to 10 days and then transferred to plastic pots containing a 3:1 mixture of potting soil and vermiculite. They were grown in a growth chamber (Aralab, Portugal) at 22 °C with a 16 h/8 h light/dark photoperiod (light provided by cool-white fluorescent tubes at a light intensity of approximately 80–100 μE/m2/s). For the analysis of promoter activity, seeds were either maintained in darkness at 25 °C (dark-grown plants), or in chambers as indicated above (light-grown plants), and collected after 3 and 10 d. Light-grown plants were transferred to pots at 12 d, and different organs were collected throughout development in order to perform GUS staining.
Medicago truncatula ecotype Jemalong line A17 seeds were chemically scarified with H2SO4 95% (v/v), surface sterilized with 5% chlorine solution for 2 min, placed in Petri dishes with modified Fahräeus supplemented with NH4NO3 to a final 1 mM N concentration (Fahräeus-N) , and stratified for 2 d at 4 °C in the dark before germination.
For growing plants in pots, seeds were transferred to a mixture of soil:vermiculite (3:1), and grown in a chamber (Aralab, Portugal) at 25 °C with a 16 h/8 h light/dark photoperiod (light provided by a mix of cool-white and red fluorescent tubes at a light intensity of approximately 200–300 μE/m2/s).
For growing plants in trays, seeds were germinated on a glass plate covered with filter paper soaked in Fahräeus-N liquid media, either in the dark (dark-grown plants) or in a chamber using the above mentioned light conditions (light-grown plants).
To perform sqRT-PCR experiments, plants were grown in the light and in darkness in trays as indicated above, and collected at 24 hpi and at 3-, 6- and 10-d-old. Roots and aerial parts were collected separately, except for 24 hpi seeds, and immediately frozen in liquid N. Different organs (roots from 30-d-old plants; leaves, flowers and pods at 3 developmental stages; and green seeds) were collected from plants grown in pots upon maturity and immediately frozen in liquid N. The developmental stages of flowers and pods are based on Kurdyukov et al. , and all of the stages analysed are fully described in Additional file 3. Green seeds were collected at the end of the filling phase (24–26 d after pollination) according to Verdier et al. . At that time, the water content in seeds has decreased below 50% and desiccation tolerance is increasing exponentially .
For the chemical treatments, germinated seeds were kept in trays with Fahräeus-N for 6 d at the usual conditions of light and temperature, and then transferred to a new tray for 24 h with Fahräeus-N supplemented with: abscisic acid (ABA, 100 μM); benzylaminopurine (CK, 10 μM); epibrassinolide (BL, 10 μM); ethephon (ET, 1 mM); giberellic acid (GA, 100 μM); indolacetic acid (IAA, 10 μM); mannitol (250 mM); methyl jasmonate (MeJA, 100 μM); salicylic acid (SA, 1 mM); sodium chloride (NaCl, 150 mM); strigolactone GR24 (SL, 10 μM); as well as a mixture of MeJA with one of the following: ET, GA and SA, at the same concentrations as when individually applied. Temperature treatment was carried out for 12 h in darkness at 4, 25 and 37 °C, with 25 °C as the control temperature. Finally, for N (−N) and Pi (−Pi) starvations, plants where kept for 7 days either in Fahräeus without N supplement or Fahräeus-N media without phosphate, respectively. Wounding was performed on one foliole by transversally cutting the middle vein.
Cis-regulatory element search and promoter analysis
The promoters of three M. truncatula ST genes (pST1, pST2 and pST3) were examined. Analyses were conducted using 1000 bp upstream of the ATG start codon of each gene. The transcript initiation site and the presence or absence of TATA boxes within the pST promoters were studied with the TSSP Software (Softberry Inc) using the default parameters. Searches for known CREs were conducted using PLACE  and PlantCARE . Elements found in both direct and complementary chains of the DNA sequences were considered. All elements retrieved in the searches were taken into account except for the TATA boxes, matrix/scaffold attachment regions or TATA-less promoters transcription initiation motifs. Databases were scanned in the order shown above, and only non-redundant elements were considered for PlantCARE. Core elements were only considered when not overlapping with another specific CRE. The percentage of each category was calculated with respect to the total number of CREs found per promoter.
Promoter and ORF cloning
A search for the M. truncatula ST gene family promoter region sequences was done using phytozome v9.1. The promoter region of each ST gene analysed, consisting of a sequence of ca. 2100–3000 bp upstream from the translational start site, was PCR-amplified (Kapa HiFi HotStart polymerase by Kapa Biosystems, USA) from M. truncatula gDNA, adding the attB1 and attB2 sequences to the 5′- and 3′-terminals for Gateway™ cloning. Additionally, a shorter version of pST3 (pST3·F2 of 1073 bp) was cloned because in this version of the M. truncatula genome sequence there was a putative ORF 1 kb upstream of the ST3 ORF. To obtain gDNA, the leaves of 40-d-old plants grown in pots were collected, immediately frozen in liquid N and ground using a MM 400 Mixer Mill (RETSCH, Germany). Two hundred mg of the ground sample were used to purify gDNA using Qiagen DNeasy Plant Mini kit (Qiagen, USA).
To clone ST1 [EMBL:LN827607], ST2 [EMBL:LN827608] and ST3 [EMBL:LN827609] ORFs, RNA was extracted from roots. One hundred mg of each sample, obtained as indicated above, were used to obtain RNA using Nucleospin® RNA plant kit (Macherey-Nagel, Germany). To avoid RNA degradation all instruments were deep washed with the RNase inhibitor RNaseZap® solution (Ambion, USA).
Several primer pairs were designed for each promoter and each ORF using the online tool at  and those that allowed the cloning of the promoters (primers 1 to 7) and the ORF (primers 8 to 14) are listed in Additional file 5. The amplified products were gel-purified (NucleoSpin® Gel and PCR Clean-up by Macherey-Nagel, Germany), checked for the correct size and sequenced. Several attempts were made to clone the ST1 ORF because the size of the expected PCR product, based on the deposited sequence, and the size of the obtained fragment were different. In fact an extra primer from 3′ UTR was used (number 3 in Additional file 5) in order to assure that the right ST1 ORF had been cloned.
Arabidopsis thaliana Transformation
The pST::GUS and p35S::ST-GFP reporter gene cassettes were prepared using Gateway™ cloning technology (Invitrogen, USA) according to the manufacturer’s instructions.
The cloned regions of each ST were used in the BP reaction with pDONR201 (Invitrogen, USA). pENTR201.pST and pENTR201.ST vectors were selected by PCR (primers 15 and 16 in Additional file 5), and sequenced to check for both correct cloning and sequence. The entry clones generated with cloned pST or ORF were used in the LR reaction with pKGWFS7, a C-terminal GFP/GUS destination vector, and pK7FWG2, a vector that generated GFP-fused proteins at C-terminal end driven by p35S, respectively, to make the corresponding expression vectors (for more characteristics see ) . All transgene cassettes in expression vectors were verified by PCR using two pairs of primers, one of them that annealed in the cloned sequence (primers 1 to 14, Additional file 5) and the other in the vector (primers 17 and 18, Additional file 5).
All expression vectors generated were electroporated into Agrobacterium tumefaciens strain C58C1m, and the A. thaliana ecotype Col-0 was transformed using the Agrobacterium-mediated floral dip method .
Seeds harvested from infiltrated plants were screened using the appropriate antibiotic and resistant seedlings (T1) were selected. T2 plants were used for analysing promoter activities by the GUS assay and subcellular ST localization by confocal microscopy.
GUS staining using X-glucuronide (5-bromo-4-chloro-3-indolyl-b-D-GlcUA) (Duchefa, The Netherlands) was performed as described by Albornos et al. .
Plant material was incubated overnight at 37 °C in GUS staining solution (5-bromo-4-chloro-3-indolyl-β-D-GlcA, 50 mM Na phosphate buffer pH 7.0, 2 mM potassium ferrocyanide, 2 mM potassium ferricyanide and 0.2% Triton X-100) and GUS-stained tissues were cleared in 70% ethanol.
GUS activity was assayed in 3 and 10-d-old seedlings grown on MS plates (both light- and dark-grown seedlings) and in the different organs of 12 to 38-d-old plants growing in the potting soil and vermiculite mixture, as already mentioned. Images were acquired using a Leica M205 FA stereomicroscope equipped with a Leica DFC495 camera (Leica Microsystems, Germany).
Experiments were performed in at least two plants of three independent T2 lines. Only when several independent transgenic lines displayed the same pattern of expression of the reporter gene was it consider as positive. Wild-type control plants showed no GUS activity (data not shown).
Semi quantitative reverse-transcription polymerase chain reaction (sqRT-PCR)
First-strand complementary DNA (cDNA) was synthesized from 500 ng of RNA extracted as indicated above by priming with a mixture of hexanucleotides (final concentration 20 μM) plus oligo dT (final concentration 2.5 μM) using PrimerScript™ RT reagent kit (Takara Bio Inc.) following the manufacturer’s instructions. Once obtained, the cDNA was diluted 10 fold before using in the PCR analysis.
Because of the high sequence similarity existing among the ST genes, it was necessary to verify that the primers designed, flanking the intron sequence found in ST genes, only recognized the transcript of interest. Several primer pairs were tested in both gDNA and cDNA, as well as in the ORF previously cloned. The specific primers used are listed in Additional file 5 (numbers 19 to 24). A phosphatidylinositol 3- and 4-kinase belonging to ubiquitin family (Medtr3g091400 gene, TC102473) tested by Kakar et al.  was used as an internal standard, and the PCR primers used for its amplification are also listed in Additional file 5 (numbers 25 and 26). The fragments amplified using cDNA as a template ranged from 150 to 250 pb.
PCR was performed by GoTaq® DNA polymerase (Promega, USA) using 2 μl of 1:10 diluted cDNA in a standardized protocol of: 1 × 95 °C for 5 min; 32 × 95 °C for 1 min, 60 °C for 1 min, 72 °C for 30 s; and 1 × 72 °C for 5 min. Images of the ethidium bromide-stained agarose gels were acquired with a ChemLite 400 FA (Avegene Life Science, Taiwan) (Additional file 4) and quantification of the bands was performed by TotalLab Quant 1D gel analysis v.10. Quantification of ST amplicons was done relative to the ubiquitin band, which was given an arbitrary value of 100 units. Band intensity was expressed as normalized and integrated absorbance (nIA). RNA was obtained from two independent biological replicates, and two cDNA synthesis reactions of each RNA were performed. Every cDNA template was used for sqPCR at least twice. Mean and standard deviation of all experiments were calculated. Statistical analyses were carried out using IBM SPSS statistics v23. When comparing transcript levels of a given ST gene among organs and developmental stages a one-way ANOVA followed by Scheffe’s test was performed, significant variations were considered for p-values less than 0.05. When treatments were compared to a control, ANOVA followed by Dunnett’s test were performed to find variations. Significant variations were considered for p-values less than 0.05, being: * 95% confidence level (p-value < 0.05); ** 99% confidence level (p-value < 0.001); and *** 99.9% confidence level (p-value < 0.05).
Roots of 22 to 25-d-old T2 transgenic plants having ST1, ST2 or ST3 ORFs fused to GFP were observed by confocal microscopy (LEICA DMI-6000B equipped with confocal SP5 system). Before visualization roots were mounted either in water or in 0.6 M mannitol in order to plasmolyse the cells. Cell wall staining was performed by immersing the roots in a 10 μg/ml PI solution for 2 min, followed by extensive rinsing with distilled water.
ABA responsive element
Cis-acting regulatory element
Domain of unknown function
Expressed sequence tag
Green fluorescent protein
Hours post imbibition
Late embryogenesis abundant
Light regulated elements
Murashige and Skoog media
Medicago truncatula gene expression atlas
Normalized and integrated absorbance
Open reading frame
Transgenic Arabidopsis plants carrying p35S::ST-GFP transgene
- pST :
Promoter of Medicago truncatula ST gene
Transgenic Arabidopsis plants carrying pST::GUS transgene
Semi quantitative reverse-transcription polymerase chain reaction
Seed storage protein
ShooT specific/Specific Tissue
Transcription start site
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We thank Dr. Beltrán’s Laboratory (IBMCP, UPV) for providing the barrel medic seeds and protocols.
This work was funded by the Spanish Ministry of Science and Technology (BFU2013–44793-P). Lucía Albornos was supported by a Training of University Teachers Grant (FPU) from the Spanish Ministry of Education, Culture and Sport.
Availability of data and materials
Promoter and ORF sequences in this study can be obtained in the Phytozome database  and the cloned ORFs in the EMBL database under accession number [EMBL:LN827607] for ST1, [EMBL:LN827608] for ST2 and [EMBL:LN827609] for ST3. Other data sets supporting the results of this article are included within the article and the Additional files.
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Classification of cis-acting regulatory elements found in ST1, ST2 and ST3 promoters under different categories and subcategories. (DOCX 29 kb)
List of all cis-acting regulatory elements found in pST1, pST2 and pST3 promoters using the PLACE and the PlantCARE databases. (XLSX, 29 kb)
Medicago truncatula plants along its development. (PDF 3399 kb)
Agarose (2%) gel electrophoresis of sqRT-PCR products. (PDF 850 kb)
Primers used in PCR amplifications. (DOCX 78 kb)
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Albornos, L., Martín, I., Labrador, E. et al. Three members of Medicago truncatula ST family are ubiquitous during development and modulated by nutritional status (MtST1) and dehydration (MtST2 and MtST3). BMC Plant Biol 17, 117 (2017) doi:10.1186/s12870-017-1061-z
- Abiotic stress
- cis-acting regulatory elements
- Medicago truncatula
- ST protein